Mycobacterium tuberculosis infection described in the present. Mutations in the rpob gene of rifampinresistant mycobacterium tuberculosis isolates in spain and their rapid detection by pcrenzymelinked immunosorbent assay. For mycobacterium tuberculosis, the rifamycinresistant mutations most commonly encountered involve codons 516, 526, and 531 numbered, by convention, as in escherichia coli rpob. Mutation analysis of mycobacterial rpob genes and rifampin. Pdf mutations in the rpob gene of rifampicinresistant. Identification of a mycobacterium tuberculosis gene that enhances. Pdf mutations in the rpob gene of multidrugresistant. Molecular characterization of the rpob gene mutations of mycobacterium tuberculosis isolated from china article pdf available in journal of tuberculosis research. The remaining isolates searched also had no mutations in the nterminal region of this gene. We evaluated the mutations in a 193bp of the rpob gene by automated sequencing of rifampicin rmpresistant and susceptible mycobacterium tuberculosis strains isolated from brazil 25 strains and france 37 strains. Rapid detection of mutations in rpob gene of rifampicin resistant mycobacterium tuberculosis strains by line probe assay article pdf available in the indian journal of. Pdf mutations in the rpob gene of rifampinresistant. Pdf characterization of rpob mutations in rifampin. Visual summary, gene names, and structures of the molecular determinants of.
Availability of an assay for detecting mycobacterium. Along with clinical, radio graphic, and other laboratory findings, results of the assay aid in the diagnosis of pulmonary tuberculosis tb. The former has not yet been cultured on artificial media. These mutations result in high rifampicin resistance with a relatively low loss of fitness. A total of 196 rifampicin rmp resistant mycobacterium tuberculosis isolates were searched for mutations in the cluster i region of the rpob gene. Complete genome sequence of mycobacterium tuberculosis k from. Characterization of rpob mutations in rifampinresistant clinical mycobacterium tuberculosis isolates from greece article pdf available in journal of clinical microbiology 361.
Fiftythree mutations of 18 different kinds, 17 point mutations and one deletion, were observed in 43 of 44 resistant isolates. Mycobacterium tuberculosis molecular determinants of. Seventeen different types of mutations were observed in 184 93. Mycobacterium tuberculosis typus humanus lehmann and neumann 1907 mycobacterium. Molecular characterization of the rpob gene mutations of mycobacterium tuberculosis isolated from china article pdf available in journal of tuberculosis research 0101. Mtbc could have a protective role against active tb disease and also against other pathologies.